The conditions you have to meet are specific to the color-coded spaces. For example, if it provides a single number, every side of a tile in that space must add up to the number provided. It is possible – and common – for only half a tile to be within a color-coded space.
In some cases, alternative gelling agents might provide faster results. Researchers observed that bacterial cellulose and another bacterial polysaccharide, Eladium, allow a 50 percent increased growth rate for various bacteria and yeasts (as compared to their growth on agar), including higher biomass yields or faster detectable biofilm formation. However, both substances are still not as cheap and readily available as agar.
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